IRCCS San Raffaele Scientific Institute in Milan, Italy, is looking for a Research Fellow to join a newly established research line under the supervision of Michela Milani, Project Leader in the lab led by Alessio Cantore (https://tinyurl.com/muj89n8d) at the San Raffaele Telethon Institute for Gene Therapy (SR-Tiget).
We are looking for a motivated Research Fellow to work on an exciting new project aiming at developing an innovative in vivo lentiviral vector gene therapy strategy for hemophilia A. In particular, we will develop different lentiviral vectors encoding for engineered version of human factor VIII (FVIII) transgene under the control of different megakaryocyte-specific promoters. These vectors will be tested in vitro and in vivo in hemophilia A mouse models, to select the best candidate to achieve effective FVIII expression in platelets after in vivo gene therapy to hematopoietic stem and progenitor cells. This project stems from our solid and extensive experience in in vivo gene therapy for hemophilia and our recent discovery of a window of opportunity in newborns to target in vivo hematopoietic stem and progenitor cells by intravenously administered lentiviral vectors.
Main activities:
Molecular cloning of new LV constructs targeting FVIII expression to megakaryocytes
Production and in vitro characterization of recombinant lentiviral vectors
In vivo testing in mouse models of hemophilia A of the generated LV
Immunological assays to evaluate immunogenicity of the developed strategy
Data analysis and critical interpretation of the results
Discussion of project results at internal and external meetings.
Who we are looking for:
Degree in biology/biotechnology (top grades) and research experience in competitive laboratories
The selected candidate is expected to apply for a Ph.D. program in the future
Cell culture and molecular biology experience
Previous experience in work with mice is considered a plus
High accuracy, reliability, precision
High motivation to work in an international environment
Excellent organizational skills
Friendly, collaborative, pro-active mindset
Good oral and written English skills.
Work details:
Type of contract: fixed-term contract
Duration: 1 year renewable;
Place of work: Milan, San Raffaele Institute.
Competitive salary depending on qualifications and experience.
At San Raffaele Scientific Institute, a world-renowned center of excellence for clinical and scientific activities, we believe that considering diversity and promoting an open and inclusive environment is crucial to our success. We are committed to ensure that equal employment opportunities are provided and based on merit without regard to age, religion, sex, disability, medical condition, sexual orientation, cultural and social backgrounds, gender identity or expression, national or ethnic origin, or any other legally recognized status entitled to protection under applicable laws. We offer attractive conditions and rewards appropriate to an internationally competitive research organization.
